In this paper we present the bone repairing immediately by ostrich eggshell powder that performed powder by ultrasonic way, lower than bone healing by self healing. The radiological and clinical survey about bone healing by ostrich eggshell powder was compared with the result of self-healing of bone defect in control groups. The study was performed on 10 mix bread dogs in two groups. Two defects on tibia diaphysis bone has been done, that one of them filled by ostrich eggshell powder and another one defect was empty for self-healing as control. Clinical and radiological evaluation of the animal model were done at thirty days and sixty days after beginning of study. Radiologically finding seems to be satisfactory regeneration with acceptable new bone formation due to osteogenesis in the main defect that filled by ostrich eggshell powder at earlier stage and bone healedremodeled defect sites that filled by ostrich eggshell powder at 60th day. Minimalbone reconstruction in the control defects are seen in the 30th and 60th day.Surgical complication in the animal study hind limbs didn't seen. Body weights were normal. Physical examination detected ostrich eggshell particles remnants under the skin was evidenced, Deformity and new bone formation on the tibia bones didn't see. Bone healing by Ostrich eggshell that powdered by ultrasound way was helpful compared with natural bone healing. There may be a significant relationship between particle size and bone oste induction that requires additional testing to determine this relationship. The reaction to ostrich egg shell particles was a non-immunogenic reaction without destructive effects.