This study focuses on evaluating the ameliorative effect of melatonin and vitamin C administration alone or in combination on haemotoxicity of acrylamide in male rats. Sixty adult male Wistar rats divided into six groups of (10 animals in each group) divided randomly into two periods, two groups in first period C (control group), 20 male rats given 5ml of distal water and Acr (ACR group), forty male rats given ACR (0.5µg/kg BW/day) for 60 days. After 60 days the groups in the first period divided into five groups, C1 (reasonable control) give distal water orally, Acr-group, given distal water orally, Mel (5mg/kg BW/day), Vit.C-group (200 mg/kg BW/day), Mel+Vit.C-group (5+200 mg/kg BW/day) were administered by gavage once daily. At the end of the administration period, the rats were anesthetized, then the blood samples collected and analyzed. The results appeared the enhancing role of melatonin to the toxicity induced by acrylamide more than to other treatment groups in both RBCs and WBCs parameters.