The objective was to assess the consequence of tris-extender enriched with different concentrations of  Royal jelly on buffalo bull semen preservability. Pooled semen was diluted with tris (control, 0% Royal jelly) and variable concentrations of Royal jelly in tris (TR) ( 0.05, 0.1, 0.2, 0.3 and 0.4%). Diluted semen was exposed to semen freezing procedures. Semen assessment was carried out for both cooled and frozen semen.In cooled semen, sperm parameters were maintained as the control. The second concentration gave the best sperm membrane integrity(HOST) percent, while the lowest was at the 0.4% concentration. Acrosome integrity was the highest in the 0.1% concentration ..
Sperm motility of post-thawed frozen semen exhibited that, the superior was given with the 0.05, 0.1and the 0.2% concentrations of royal jelly while ,the lowest was at the last two concentrations(0.3 and 0.4%). Alive sperm percent was maintained in all the concentrations as the control .The HOST percent was significantly higher in the five concentrations and the best was given with the 0.05%concentration . It is concluded  that ,in cooled semen  the 0.1%concentration gave the best sperm quality, while the lowest was at the 0.4%concentration.. Sperm parameters of post-thawed frozen semen exhibited that, the superior was given with the 0.05%, 0.1%and the 0.2% concentrations of royal jelly while ,the lowest was at the last two concentrations(0.3 and 0.4%).  . Conception rate was the best in Tris royal jelly (TR₁,TR₂ and TR₃) and decreased at the last two concentrations.