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98784

Morphological and Molecular Characterization of Dicrocoelium spp. Isolates from Sheep, Goat and Cattle in the West of Iran

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Veterinary Parasitology and Bacteriology

Abstract

DICROCELIOSIS is a prevalent parasitic disease in ruminants and in humans and is accidental caused by different species of Dicrocoelium spp. This study aimed to evaluate the morphological characteristics and molecular parameters of Dicrocoelium spp. isolated from livers of slaughtered sheep, goat, and cattle in three provinces in west of Iran from Feb 2018 to Feb 2019. In the present study, 45 infected livers with Dicrocoelium were collected (each province 15 and each host 5 infected liver). After morphological examination, the molecular evaluation was carried out for the ribosomal DNA (partial 5.8S, complete ITS2 and partial 28S genes) using PCR and PCR-RFLP techniques. Morphological analysis revealed that all specimens belonged to Dicrocoelium dendriticum. There were profound morphometric variations among D. dendriticum from different hosts and sampling areas, but no statistically significant differences was found (P>0.001). The sequencing analysis of obtained fragments in GeneBank showed 96% to 100% similarity within homogeneous fragments in the database. The obtained sequences were also registered in Gene Bank with the accession numbers, MN831473, MN831474 and MN831475. Additionally, all PCR-RFLP products revealed two similar fragments (319bp and 271 bp) in all examined samples. According to the present study, haplotype A was shown to be the predominant haplotype of D. dendriticum in the west of Iran.
 

DOI

10.21608/ejvs.2020.29595.1174

Keywords

Dicrocoelium, ITS2, Iran, Morphology, PCR-RFLP

Authors

First Name

Mohammad

Last Name

Ebrahempour

MiddleName

Hossein

Affiliation

Department of Pathobiology, Sciencep and Research Branch,Islamic Azad University, Tehran, Iran

Email

hossein.ilam2011@gmail.com

City

ilam

Orcid

0000-0002-1683-7150

First Name

bahar

Last Name

shemshadi

MiddleName

-

Affiliation

Department of Pathobiology, Scienc and Research Branch,Islamic Azad University, Tehran, Iran

Email

bshemshadi@yahoo.com

City

Tehran

Orcid

0000-0001-8996-3062

First Name

Ali

Last Name

Bahrami

MiddleName

Mohammad

Affiliation

Department of Pathobiology, Faculty of Veterinary ParaMedicine, Ilam University, Ilam, Iran

Email

am.bahrami@ilam.ac.ir

City

ilam

Orcid

-

First Name

Salome

Last Name

Shirali

MiddleName

-

Affiliation

Department of Biotechnology, Faculty of Basic Sciences, Ahvaz Azad University, Ahvaz, Iran

Email

s.shirali2017@gmail.com

City

Ahvaz

Orcid

0000-0003-2748-3236

Volume

51

Article Issue

3

Related Issue

14474

Issue Date

2020-09-01

Receive Date

2020-05-07

Publish Date

2020-09-01

Page Start

321

Page End

328

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 51_Issue 3_Pages 321-328.pdf

PDF . 1.4MB

Link

https://ejvs.journals.ekb.eg/article_98784.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=98784

Order

4

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Morphological and Molecular Characterization of Dicrocoelium spp. Isolates from Sheep, Goat and Cattle in the West of Iran

Details

Type

Article

Created At

22 Jan 2023