The aim of the present study was to explore the effect of Moringa oleifera leaves extract (MLE) on buffalo spermatozoa during cooling and cryopreservation. Pooled bull semen were diluted by Tris-Citrate-Fructose (TCF) diluent supplemented with MLE and allocated in 6 concentrations which were 0% control, 10, 20, 30, 40, 50% (v/v) [MLE: TCF] then 20% egg yolk was added, mixed and finally stored at -20°C until used. Diluted semen was cooled slowly up to 5 °C and equilibrated for 4 h. Semen was packed into 0.25 mL polyvinyl straws. After equilibration periods, the straws were exposed to liquid nitrogen (LN2) vapor for 10 minutes and were then dipped in LN2. Extended semen was subjected to evaluation (motility, alive %, abnormality %, and intact sperm membrane (HOST) %) in both chilled and frozen semen. Sperm motility was significantly (P<0.0001) kept high after 11 days of chilling with the concentration 10 and 20%, also it was kept significantly (P<0.0001) high with the concentration 30 and 40% up to 10 days. The use of MEEY had significantly (P<0.0001) improved sperm motility% with the concentration 10 and 20% compared to the control, while the other concentrations didn't exhibit any effect. On the other hand, MEEY had maintained alive sperm, intact spermatozoa membrane% and abnormal sperm % as good as the control with all the concentrations of MEE used in the present study. Then, it could be concluded that 10-20% of Moringa oleifera leaves extract (under the contemporary conditions of extraction in this study) as a natural additive to semen extenders improved preservability in both chilled and frozen bull semen.