im: the aim of this study is to use serological diagnosis for rapid detection of Toxoplasma gondii infection among household cats. Material and Methods: 212 serum samples collected from household cats of different sex, breeds and age groups in urban and rural area of Giza Province. Samples were checked by rapid chromatographic immune assay (IC) for detection of IgM and IgG antibodies against Toxoplasma gondii. Statistical analysis of the obtained results were carried out using Chi-square test to study the effect of sex, breed and age on the results. Results: the IgM antibodies against Toxoplasma gondii were detected in 19 cats (8.96%), and IgG antibodies were detected in 63 cats (29.71%), the overall positive were 82 cats (38.67), the sero prevalence against Toxoplasma gondii was high in Egyptian Mau breed and in cats of  over one year age. Statistical evaluation of the results proved that there were significant differences between different sex, different cat breeds and different age groups. Conclusion: This study concluded that the serological diagnosis by IC for detecting IgM and IgG against Toxoplasma gondii, is a rapid, accurate method for diagnosis of Toxoplasmosis