The present work was undertaken to study the incidence of β-haemolytic streptococci infection in equine. On clinical examination of  a community of 524 Arabian  foals and horses in Cairo – Egypt, a total of 164 animals were selected showing respiratory signs (31.30 %) of which 62 (11.83  %) showed respiratory signs and lymph nodes abscessation. Two hundred and twenty six  swabs,164 nasal,31submaxillary lymph node pus and 31 retropharyngeal lymph node pus, were collected from 164 foals and horses showing respiratory signs and/ or abscessed lymph nodes  for Streptococci isolation and PCR confirmation.
A total of 150 isolates of Streptococci were recovered from 226 samples with sample-wise prevalence of (66.37%). Out of these 150 isolates, 124(28.67%) were identified as Streptococcus equisubsp. equi, 26(17.33%) as S. equisubsp.zooepidemicus and no S. dysgalactiae subsp. Equisimilis were identified.
The incidence of S. equisubsp.Equi and S. equisubsp.Zooepidemicus infection among the total animal population, in the present study, was 11.83 and 4.96 % respectively. PCR technique showed high  sensitivity and specificity  for the detection of S. equi species in the examined  samples.