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Isolation and Identification of H9N2 Avian Influenza and Newcastle Disease Viruses co-Infections in Chicken

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Last updated: 01 Jan 2025

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Abstract

Co-infections with Newcastle disease (ND) and avian influenza (AI) highly pathogenic viruses causes high mortality and high economic losses. In this study, 322 samples were collected from 50 chicken flocks suffering from respiratory symptoms, severe drop in egg production and mortalities from December, 2013 to December, 2014. Real time polymerase chain reaction (RT-PCR) was performed to detect the M protein of H9 gene of AI and F gene of velogenic ND virus (vNDV) co-infection. H9 PCR was 20 and 34 in cloacal and pharyngeal swabs with total percentage of 6.2 % and 10.5%, respectively. The H9 sequences showed low pathogenic RSSR/GLF motif. The phylogenetic tree showed that H9 sequences belong to the G1 lineage which clustered with 2015 -2016 Egyptian H9N2. Partial sequences of the Fusion gene revealed that the isolated NDV isolates belong to class II genotype VIIj.  The cleavage site of the F protein showed the presence of a polybasic amino acid motif (112RRQKRF117) suggesting that our two isolates were vNDV strain. The molecularly identified H9 (1 isolate) and NDV Genotype VII (2 isolates) were named AI/CHICKEN/EGYPT/48Ob/NRC-CU/2014(H9), NDV/CHICKEN/EGYPT/48Ob/NRC-CU/2014, and NDV/CHICKEN/EGYPT/ 66Oc/NRC-CU/2014, and nucleotide sequences were submitted to GenBank and given accession numbers MG966504, MG966505, and MG966506, respectively. Conclusion: Natural mixed infections with AIV H9N2 and virulent NDV genotype VIIj viruses were detected in chicken flocks suffering from respiratory symptoms and mortalities in Egypt. 

DOI

10.21608/ejvs.2018.4963.1043

Keywords

H9N2, NDV genotype VII, co-infection, RT-PCR, Phylogenies

Authors

First Name

Mohamed

Last Name

Amer

MiddleName

Mahrous

Affiliation

Department of Poultry Diseases. Faculty of Veterinary Medicine. Cairo University. Postal Code: 12211 Giza, Egypt.

Email

profdramer@yahoo.com

City

Giza

Orcid

0000-0001-8965-7698

Volume

49

Article Issue

2

Related Issue

934

Issue Date

2018-12-01

Receive Date

2018-08-29

Publish Date

2018-12-01

Page Start

135

Page End

146

Print ISSN

1110-0222

Online ISSN

2357-089X

Article File

EJVS_Volume 49_Issue 2_Pages 135-146.pdf

PDF . 672.8kB

Link

https://ejvs.journals.ekb.eg/article_15307.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=15307

Order

7

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Isolation and Identification of H9N2 Avian Influenza and Newcastle Disease Viruses co-Infections in Chicken

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Type

Article

Created At

22 Jan 2023