H EPATOTOXICITY is the major health problem that is a global concern worldwide especially in Egypt because the liver injury is one of the most ten disease leading to death. There are many factors leading to hepatotoxicity as antibiotics like Isoniazid (INH) and rifampicin (RIF) which are the first line remedies in treatment of tuberculosis. The present study evaluates the possible hepatoprotective effects of Moringa oleifera against the experimentally induced hepatotoxicity with INH/RFP in rats. Forty eight rats (200-250g) were allocated into six groups (8 rats in each group), and treated as follow: group I: received normal saline orally, group II: received INH/ RIF (50 mg/Kg/day of each)for 28 days orally, group III: received Silymarin (50 mg/kg/day) + INH/ RIF for 28 days orally, group IV, V, VI: received Moringa oleifera ethanolic extract (MOEE) (250, 500 and 1000 mg/Kg/day) + INH/ RIF for 28 days respectively. Co-administration of Moringa oleifera with INH/ RIF reduced elevated serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP) and (bilirubin total &direct) levels but also decreased the elevated malondialdehyde (MDA) & tumor necrosis factor-α (TNF-α) contents in liver homogenate. Moreover, administration of Moringa oleifera increased glutathione peroxidase (GPX) & adiponectin activity. Microscopic examination of Moringa olifera extract administration revealed reduction in the severity of liver damage. It is concluded that Moringa olifera might be considered as adjuvant drug in treatment of liver disorder and or as hepatoprotective therapy with anti-tubercular drugs.