Artificial insemination by frozen semen is a major approach for diseases transmission. This work intended to scan the buffalo bull semen for brucellosis caused by Br. melitensis, toxoplasmosis caused by Toxoplasma gondii and Neosporosis caused by Neospora caninum using Polymerase Chain Reaction (PCR). Fifty bulls were used in this study. Semen was assessed for volume, motility, viability, and chromatin damage. Total genomic DNA was extracted from buffalo semen and amplified by PCR. The results showed that, semen samples were positive to toxoplasmosis in 6% of the semen samples, while all the semen samples were totally negative to brucellosis and neosporosis. No significant differences were found in the semen characteristics between bulls positive and that were negative to toxoplasmosis.
In conclusion, the present trial suggests the opportunity of venereal transmission of T. gondii among buffaloes .However, further investigations are required using high number of semen samples from different localities of Egypt.