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Detection of virulence genes of Salmonella in diarrhoeic ducks by using Polymerase chain reaction

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Last updated: 01 Jan 2025

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Abstract

Salmonella spp. is responsible for various foodborne outbreaks. Incidence of Salmonella among the examined diarrheic ducks was 5.5%. The occurrence was 6.15% in young ducks which was higher than that in adult duck 4.28%. The total incidence of Salmonella in slaughtered ducks was 3.33%. The serovars were S.Infants, S. Typhimurium , S. Virchow from fecal samples of adult ducks and S. Agona, S. Infants, 2 S. Kentucky, 2 S. longhorn, S. Typhimurium and S. Virchow in fecal samples of young ducks while from slaughtered ducks were (S. Kentucky and S. Typhimurium from both caeci and intestines and S. Typhimurium from livers ).
Variation in pathogenicity and enterotoxin production of Salmonella isolates were observed.
Sensitivity of salmonellae to ciprofloxacin, flumequine were 56.3%, enrofloxacin , gentamicin and norfloxacin were 50% and trimethoprim was 18.8%. All isolates were resistant to doxycycline hydrochloride and penicillin .
All isolates from diarrheic and slaughtered ducks harbor invA gene and amplified at 284bp. Sixteen Salmonella isolates examined for pef gene at 700bp that was present in 2 S. Typhimurium, S. Agona and S. Kentucky isolated from diarrheic ducks and 2 S. Kentucky isolated from slaughtered ducks. Finally we investigate the presence of stn gene that was encoded on plasmid DNA and amplified a region 617bp in 16 isolates of Salmonella 2 S. Typhimurium, 2 S. Virchow, S. Agona, S. Infant, 2 S. Kentucky and S. longhorn isolated from diarrheic ducks while 2 S. Kentucky isolated from slaughtered ducks harbored this gene.

DOI

10.21608/ejvs.2017.1163.1013

Keywords

Salmonella, Ducks, virulence genes invA, pef, PCR

Authors

First Name

amany

Last Name

elgohary

MiddleName

H.

Affiliation

Amany H. Elgohary

Email

dr.amanyelgohary@yahoo.com

City

-

Orcid

-

First Name

Azza

Last Name

Abuelnaga

MiddleName

S.

Affiliation

Azza S.M.AbuElnaga2

Email

azzaabuelnaga@yahoo.com

City

-

Orcid

-

First Name

Hala

Last Name

Ibrahim

MiddleName

S.

Affiliation

Hala S. Ibrahim

Email

halasultan_65@yahoo.com

City

-

Orcid

-

First Name

Riham

Last Name

Hedia

MiddleName

H.

Affiliation

Riham H.Hedia

Email

rihamhassan1950@yahoo.in

City

-

Orcid

-

First Name

Sohad

Last Name

dorgham

MiddleName

-

Affiliation

Sohad M. Dorgham

Email

sohad_dorgham@yahoo.com

City

-

Orcid

-

First Name

Elgabry

Last Name

Elgabry

MiddleName

Abdelalim

Affiliation

E.A. Elgabry

Email

elgabry73@hotmail.com

City

-

Orcid

0000-0002-0843-0035

Volume

48

Article Issue

1

Related Issue

626

Issue Date

2017-06-01

Receive Date

2017-05-26

Publish Date

2017-06-01

Page Start

11

Page End

21

Print ISSN

1110-0222

Online ISSN

2357-089X

Link

https://ejvs.journals.ekb.eg/article_3994.html

Detail API

https://ejvs.journals.ekb.eg/service?article_code=3994

Order

2

Type

Original Article

Type Code

140

Publication Type

Journal

Publication Title

Egyptian Journal of Veterinary Sciences

Publication Link

https://ejvs.journals.ekb.eg/

MainTitle

Detection of virulence genes of Salmonella in diarrhoeic ducks by using Polymerase chain reaction

Details

Type

Article

Created At

22 Jan 2023