The influence of Chitosan (C6H11NO4)n with different molecular weights along with the Chitosan Nano-particles were studied, as supplements and alternative to mineral fertilizers due to their natural properties, on the growth and productivity of seven years-old Crimson Seedless cv. grapevine. The foliar spray was applied in order to determine the optimum rates that would promote yield quality by comparing the bulk Chitosan (CS) with the Chitosan (CS) Nano-particles. The experiment was conducted in 2019 and 2020 with a preliminary season 2018, comprised seven treatments (Control, Chitosan bulk form at 1, 5, 10 cm3/ L and Chitosan Nano-particles at 100, 150, 200 ppm). Results indicated that Chitosan (CS) Nano-particles at 200 ppm was significantly the most effective in stimulating all growth parameters followed by bulk Chitosan at 5 cm3/ L in relation to the control treatment and all other treatments. It has been shown an increase in average yield, cluster weight, berry size, shoot length, anthocyanin, TSS % and leaf pigments content besides its cost effective through reducing the amount of bulk fertilizers.