To evaluate the effect of the three citrus rootstocks: Sour orange, Rough lemon and Cleopatra mandarin on yield and fruit quality of Salustiana orange (Citrus sinensis L. Osbeck) trees a study was conducted during the two successive seasons of 2018 and 2019. The experimental trees were 12-year-old and grown in sandy soil in Wadi El-Natron region, Behira Governorate, Egypt. The obtained results indicated the Salustiana orange trees budded on Rough lemon had greater the yield / tree, fruit weight, size, fruit dimensions, pulp and peel weights/ fruit as well as peel thickness than those on Cleopatra mandarin and sour orange. Juice TSS percentage and TSS/ acid ratio of fruits for trees budded on sour orange were higher than those on rough lemon and Cleopatra mandarin.  Juice volume/ fruit for trees budded on sour orange were smaller than those budded on rough lemon and Cleopatra mandarin. While, total acidity percentage was higher in fruits of trees budded on rough lemon than those of Cleopatra mandarin. Seed number and weight/ fruit of trees budded on rough lemon reached 10.38 & 11.12 and 6.45 & 10.30 folds its values on sour orange in the both seasons. The corresponding values for trees budded on Cleopatra mandarin trees were 6.98 & 10.59 and 6.35 & 10.15 folds, respectively. This indicates that rootstock type may affect fertility of reproductive organs of Salustiana flowers, especially ovules.