This study focuses on the effects of a commercially available synthetic rooting preparation that includes chemicals of the auxin group with indolebutyric acid (IBA) and natural and organic origin biostimulant on rooting in cuttings, root and twig development. Cuttings of commercially available ornamental plant Trachelospermum jasminoides and potential ornamental plants from natural flora Hypericum calycinum and Hypericum androsaemum were used in the study. The cuttings were kept at a temperature of 24-26 ^oC and humidity of 80-85% in a greenhouse, perlite environment for 90 days.  According to the variance analysis results, the application of biostimulants in T. jasminoides was more effective in cutting rooting rates (69%), whereas there was no difference between the applications in H. calycinum (100%) and H. androsaemum (86.67%) species. As for the number of roots, all applications were equally effective in T. jasminoides (3.86 pieces) and H. androsaemum (2.94 pieces), and biostimulant was the most effective in H. calycinum (8.20 pieces). Biostimulant application in total root length, average root length, main root length, total twig length, average twig length, wet root weight data were found to be at the same level or more effective than the other applications. As a result, this study has found that natural species in flora can be successfully produced by using organic origin biostimulants instead of synthetic preparations for landscape applications.