MATERIALS of in vitro culture are the first step in most seed potato programs worldwide. Microtubers areconsiderable tool for implementationof anational seed potato production system in countries lacking isolated high cool latitude and vector free areasfor seed potato production.The current study investigatesmicrotuber production abilityof 5 genotypes imported from CIP (International Potato Center, Peru) comparing with Diamant variety. Also, studied the impact microtuberization agent(80 g/l sucrose, 5 mg/l benzyladenineor 500 mg/l Chlorocholine chloride) addedto MS medium on in vitromicrotuberization.Significant differences recorded between genotypes in microtuber weight and number. Unica and Costanera yielded the highest microtubers weight, number and average microtuber weight.The best media for microtuberization was MS supplied with 80 g sucrose. As a conclusion it is recommended to use elevated sucrose for microtubers production of Unica, Costanera, Yayla Kizi and Meva genotypes.