THIS STUDY was achieved out during two successive seasons (2015 & 2016) The experiment was conducted on 16-year-old Thompson seedless grapevines. The vines were grown in a clay soil under surface irrigation system. Three material namely Chitosan, Salicylic acid and Fulvic acid applied at rate (500 ppm) were used as a spray application either single or in combination of them on the vine for three times, at growth start, one week after berry set and at veraison as an attempt to develop growth, berry quality and enhancement storage shelf life period of Thompson seedless grapevines. The Results appeared  that single applications of Chitosan ,Salicylic acid and Fulvic acid or in combination of them were effective in improving shoot length, leaf surface area, total chlorophyll in the leaf and total protein in the canes in addition percentages of N, P and K in the leaves as well as enhancing yield per vine, cluster weight, berry weight, soluble solids content and total phenols while reducing total acidity, cluster weight loss% ,berry shatter % and berry decay% and total loss in cluster weight percentages during storage shelf life period. Also, enhanced berry adherence strength as compared with control in both seasons of study.  Application of Chitosan + Salicylic acid + Fulvic acid was superior to using applications of Chitosan, Salicylic acid and Fulvic acid alone. In addition, the best results with regard to net profit of Thompson seedless grapevines were obtained when vines were sprayed with salicylic acid + fulvic acid.