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238970

Preparation and Purification of HBsAg from HBsAg-Positive Human Blood

Article

Last updated: 01 Jan 2025

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Tags

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Abstract

HEPATITIS B virus (HBV) is one of the most serious world health problems as it is highly distributed and can result in very dangerous diseases including liver cirrhosis, liver failure or even liver carcinomas. Thus, it is very important to early diagnose it. One of the most important tools to diagnose HBV is to measure the presence of hepatitis B surface antigen (HBsAg) in patient blood. A special kit is the one of the most potent tools for this purpose, in which HBsAg is essential component. Hence, in the present study the authors prepared and purified HBsAg which can be later used as an antigen, to prepare specific antibodies and for other applications.

DOI

10.21608/ejrsa.2022.128612.1133

Keywords

Hepatitis, Hepatitis B virus, Hepatitis B surface antigen, Production of Antigen, Purification, Separation

Authors

First Name

Marwa

Last Name

Farag

MiddleName

Hany

Affiliation

Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt, [2] Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt,

Email

marwa_hany.eaea@yahoo.com

City

Cairo

Orcid

0000-0002-5280-3839

First Name

Sahar

Last Name

Shouman

MiddleName

A.H.

Affiliation

Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

sahar.shouman@sci.asu.edu.eg

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

El-Bayoumy

MiddleName

S.A.

Affiliation

Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt

Email

asami1972@mail.ru

City

Cairo

Orcid

-

First Name

Ahmed

Last Name

Abdel Mohty

MiddleName

A.

Affiliation

Radioisotopes and Generator Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt

Email

aa_moaty@yahoo.com

City

-

Orcid

-

First Name

Nagy

Last Name

Mehany

MiddleName

L.

Affiliation

Labeled Compounds Department, Hot Laboratories Center, Atomic Energy Authority, Cairo, Egypt

Email

nagy.lahzy.m@gmail.com

City

-

Orcid

-

First Name

Marwa

Last Name

Gado

MiddleName

M.

Affiliation

Microbiology Department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

marwagado@sci.asu.edu.eg

City

-

Orcid

-

Volume

34

Article Issue

1

Related Issue

34615

Issue Date

2021-12-01

Receive Date

2022-03-27

Publish Date

2021-12-01

Page Start

117

Page End

120

Print ISSN

1110-0303

Online ISSN

2314-8527

Article File

EJRSA_Volume 34_Issue 1_Pages 117-120.pdf

PDF . 998.3kB

Link

https://ejrsa.journals.ekb.eg/article_238970.html

Detail API

https://ejrsa.journals.ekb.eg/service?article_code=238970

Order

238,970

Type

Original Article

Type Code

136

Publication Type

Journal

Publication Title

Egyptian Journal of Radiation Sciences and Applications

Publication Link

https://ejrsa.journals.ekb.eg/

MainTitle

Preparation and Purification of HBsAg from HBsAg-Positive Human Blood

Details

Type

Article

Created At

22 Jan 2023