SAFEGUARDS technical measures applied on nuclear materials and activities aim at detecting and determining whether the samples are nuclear or not. For this purpose, SEM and EDX are used for fast detection of uranium and thorium within samples, but these tools stand helplessly in the case of liquid samples. Such samples need tools and further preparation to be appropriate for measurements. Graphene oxide (GO) is employed as an adsorbent for uranium because it contains various functional groups such as epoxide, carbonyl, carboxyl and hydroxyl in addition to its high specific surface area. The product of the adsorption process is measured using SEM and EDX. The as-prepared GO has been prepared then characterized using SEM, TEM, and XRD then undergone adsorption with different concentrations of uranium. The results showed that the prepared materials are efficient adsorbents for the removal of uranium from water and hence can be used to detect and determine uranium in samples.