TADALAFIL is widely used in the treatment of many disease disorders. Tadalafil is studied for its protective effect against γ-rays-induced hepatic injury in rats. Rats were exposed to 6 Gy-γ-rays then, treated with Tadalafil for 7-days and compared to the control, Tadalafil alone and irradiated groups. Oxidative-stress markers; malondialdehyde (MAD) and myeloperoxidase (MPO) and the antioxidant markers; reduced glutathione (GSH) content, and the relative enzyme activity of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were evaluated in liver tissues. The anti-inflammatory markers; tumour necrosis factor-α (TNF-α) and interleukin-6 (IL-6) were evaluated in the blood serum. Data revealed a significant reduction in the γ-rays-induced lipid-peroxidation; MDA content, MPO activity, the inflammatory markers levels of TNF-α and IL-6, and an augmentation in the antioxidant marker levels of GSH, GPx, CAT and SOD. It could be concluded that Tadalafil could alleviate the toxic effects of ionizing radiation in liver via the strong anti-oxidant and anti-inflammatory impact, thus could be useful as a radio protective agent in the radiotherapy schedule. The radio protective influence of Tadalafil could need to be explored for further organs in imminent works.