THIS STUDY was designed to investigate the protective effect of oral administration of ellagic acid (EA), a natural polyphenol (50mg/kg body wt) and/or zinc aspartate (ZA) (50mg/kg body wt) against the cellular damage induced by whole body gamma irradiation (6.5Gy as a single dose) in male albino rats. The results of the current study revealed that exposure to γ-radiation exhibited a potential elevation of serum iron, total iron binding capacity (TIBC) and transferrin; as well as lipid peroxidation (LPO) and metallothioneins (MTs) in liver and kidney. In addition, there were significant decrease in superoxide dismutase (SOD) and reduced glutathione (GSH). In addition, tissues of liver and kidney displayed changes in some trace elements concentrations. Rats treated with EA and/or ZA before and after whole body γ-irradiation revealed significant modulation of the biochemical parameters and improvement in the antioxidant status, which might be effective in minimizing the radiation-induced increase in LPO as well as changes in essential trace elements in liver and kidney tissues reflecting a synergistic effect.