Introduction: Approximately 30% of the world's population has serologic evidence of current or past HBV infection. Middle East has an intermediate level of HBV endemicity (2- 7%).It is estimated that 3% of the global population have chronic HCV infection. The prevalence of Anti-HCV in Egypt was 14 % in 2010. More than 30 dangerous blood-borne pathogens are transmitted by contaminated needles, but those of primary significance to healthcare workers (HCWs) are hepatitis B virus, hepatitis C virus and human immunodeficiency virus. Needle stick and sharps injuries (NSSIs) have been recognized as one of the most serious occupational hazards among HCWs. Aim of Work: To study the frequency and risk factors of hepatitis B & C virus infection among health care workers in general surgery department, Assiut University Hospitals. Materials and Methods: A Cross-sectional study was carried out among HCWs in general surgery department, Assiut University Hospitals. This study included 215 HCWs, of them 21 refused to be subjected to serological examination. The instrument used was a structured interview questionnaire. Blood samples were taken from HCWs to screen for Anti-HCV, HBsAg, anti-HBc and anti-HBs. Results: 5.2% of HCWs were Anti HCV positive, 3.1% were HBs Ag positive, 28.9% were Anti HBc positive, 55.7% were Anti HBs positive and 32.5% of HCWs were negative. Hepatitis virus infection was statistically significant among older age group, nurses and technicians and with long working duration. Conclusion and Recommendations: to improve hepatitis B vaccination coverage among HCWs, avoid needle stick and sharps injuries (NSSIs) and to improve the reporting system.