Background: Introduction: Vaccination of healthcare professionals is a critical issue for infection control, not only to protect them from transmissible agents, but also, to prevent nosocomial transmission of pathogens. Aim of Study:Assessment of vaccination coverage against vaccine preventable diseases among health care workers in a tertiary care hospital Riyadh region, Saudi Arabia. Materials and methods: Review of the records of staff health clinic to determine the number of health care workers fully vaccinated against each of recommended vaccines according to the Centers of Disease Control and Prevention (CDC) vaccination schedule. Results: The highest vaccination
coverage rate was for hepatitis B vaccination (95.6%) with no significant differences between different professional categories, and lowest coverage was for varicella vaccine (10.4%) with no significant difference between critical departments. Conclusion: Vaccination against hepatitis B had the highest coverage rate among all health care
categories and low vaccination rate observed for Measles, Mumps, Rubella (MMR) and varicella vaccine. Significant higher vaccination coverage of meningococcal vaccine among microbiology workers than other health care categories. Management policy and procedures should be directed and implemented to increase the vaccination coverage for recommended vaccines according to the CDC vaccination schedule to HCWs .