Asphalt fumes contain polycyclic aromatic compounds (PAC). There is a possibility of long-term health effects following chronic exposure by inhalation or skin contamination in asphalt road pavers. Objective: This study aimed at exploring the relationship between urinary 1-OH pyrene as a marker of internal dose of PAHs exposure and serum P53 protein as a response marker with the genetic polymorphism of CYP1A1 as a susceptibility marker among asphalt exposed workers. Methods: This work included 70 male individuals who were divided into an asphalt exposed group (n=43) and a matched control group (n=27). The exposed group was further subdivided into a group of paving and mixing (n=24) and a group of roller and vehicle drivers (n=19). Every participant was subjected to polymerase chain reaction (PCR) technique to detect the different genotypes of CYP1A1 gene and the expression of the p53 gene mutation. Besides, estimation of urinary 1-hydroxy pyrene (1-OHP) and serum level of mutant p53 protein was done as a PAHs exposure and response biomarkers, respectively. Results: CYP1A1*2A (Valine / Valine) was found to be the most prevalent CYP 1A1 genotype among both exposed and control groups. It was associated