IN THE PRESENT study, thirty-one isolates were collected from different locations in Tanta city, Gharbia Governorate, Egypt. These isolates were screened for their polyvinyl alcohol (PVA) - degrading ability as a sole carbon source in the culture medium. Among the thirty-one tested isolates, only Seventeen were found PVA degraders belonging to genera Penicillium, Aspergillus, Trichoderma, Fusarium and Ulocladium. The highest degradation percentages were recorded for Penicillium polonicum ON024147 (25.7%) and Aspergillus niger ON012795 (24.28%). The culture conditions were optimized for maximum PVA-degradation of 88% and 90% by P. polonicum and A. niger, respectively. The optimum static culture conditions for P. polonicum were adjusted at pH 5 and incubation at 30°C for 6 days in the presence of starch as a co-carbon source and KNO3 as a nitrogen source with PVA conc. 10g/L. For A. niger, the optimum growth conditions were at pH=6, 30°C, static culture, for 6 days, cellulose as a co-carbon source, sodium nitrate as a nitrogen source and PVA conc.= 10g/L.