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98707

Biological Control of Green Bean Damping-off Disease Caused by <i> Rhizoctonia solani </i> by <i> Streptomyces parvulus </i> Strain 10d

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Last updated: 22 Jan 2023

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Abstract

THIS STUDY aimed to evaluate the efficacy of applying spores and chitinase enzyme of Streptomyces parvulus strain 10d in biological control of damping-off disease caused by Rhizoctonia solani in green beans (Phaseulus vulgaris) and compare it with Rhizolex fungicide application. Five seeds of green bean were sown in plastic pots, filled with either sterilized or non-sterilized soils, infested with R. solani inoculum (5g/kg soil). Pots were kept in the greenhouse for 45 days from sowing. Pre-emergence damping-off was recorded 15 day from planting and post-emergence damping-off and survival rate were recorded 30 days from planting. Plants growth characteristics were recorded 45 days of sowing.
Results showed that Rhizolex treatment had the best survival rate and lowest root–rot severity (79 and 25%), followed by crude enzyme treatment (76 and 25%), then spores' treatment (75 and 27%, respectively), in infested and non-sterilized soils.
For plant growth characteristics, in infested and non-sterilized soil, spores' treatment had the best effect on plant height (41.5cm), followed by crude enzyme (39.5cm) and Rhizolex treatments (34.6cm). Numbers of leaves/plant were the highest in Rhizolex (17), followed by spores (15) and enzyme treatments (14). Numbers of pods/plant were 14 in Rhizolex treatment and 11 in crude enzyme and spores' treatments. The highest dry weight was recorded in spores' treatment followed by Rhizolex treatment (10 and 7 g/plant, respectively). 
Conclusions: results suggest that using chitinolytic Streptomyces strain 10d for the biological control of R. salani and damping-off disease of green bean plants can be an attractive alternative for pesticides in organic agriculture.

DOI

10.21608/ejm.2020.22329.1145

Keywords

biological control, Chitinase, Damping-off, Green Beans, <i> Phaseulus vulgaris, Rhizoctonia solani </i>, Rhizolex, <i> Streptomyces parvulus </i>

Authors

First Name

A.S.

Last Name

Korayem

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Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

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First Name

A.A.

Last Name

Abdelhafez

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Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

Email

aabdelhafez@yahoo.com

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Orcid

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First Name

M.M.

Last Name

Zaki

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Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

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Orcid

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First Name

E.A.

Last Name

Saleh

MiddleName

-

Affiliation

Agricultural Microbiology Department, Faculty of Agriculture, Ain Shams University, Cairo, Egypt

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Volume

55

Article Issue

The 14th Conference of Applied Microbiology

Related Issue

14972

Issue Date

2020-06-01

Receive Date

2020-01-12

Publish Date

2020-06-01

Page Start

87

Page End

94

Print ISSN

0022-2704

Online ISSN

2357-0881

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https://ejm.journals.ekb.eg/article_98707.html

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https://ejm.journals.ekb.eg/service?article_code=98707

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8

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Original Article

Type Code

121

Publication Type

Journal

Publication Title

Egyptian Journal of Microbiology

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https://ejm.journals.ekb.eg/

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Article

Created At

22 Jan 2023