98700

Safe Application and Preservation Efficacy of Low-toxic Rhamnolipids Produced from <i> Ps. aeruginosa </i> for Cosmetics and Personal Care Formulation

Article

Last updated: 01 Jan 2025

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Abstract

THE INTEREST in natural antimicrobial preservatives is rapidly grown due to their exceptional properties such as biodegradability, biocompatibility, and lower toxicity. On the contrary, common synthesized preservatives are more toxic, partially or non-degradable, and have undesirable side effects on human health and the environment. Furthermore, the resistance of microorganisms to current antimicrobial preservatives is increasing. In the present study, rhamnolipid was produced from Pseudomonas aeruginosa ATCC 9027, and twelve congeners of rhamnolipids were elucidated by ESI-MS. Additionally, rhamnolipids reduced the surface tension of water to 26mN/m with a critical micelle concentration value of 22mg/L. The produced rhamnolipids showed prominent lower toxicity against the brine shrimp Artemia salina than those of positive control and methylisothiazolinone. A significantant antimicrobial effect of rhamnolipids was achieved in vitro, whereas the disc diffusion assay showed noticeable inhibition zones around the paper discs saturated with different concentrations of the rhamnolipid produced in this study, with MICs ranged between 8 and 336μg/ml against Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Staphylococcus aureus, Bacillus subtilis, Saccharomyces cerevisiae, Candida albicans and Aspergillus brasiliensis. Moreover, a promising preservation efficacy of the produced rhamnolipid was obtained when applied to a personal care formulation model even after 28 days of incubation, with reduction values of about 3 and 1 expressed in log units for bacteria and fungi, respectively. However, rhamnolipids could be recommended as a powerful nontoxic bio-preservative for personal care formulations.

DOI

10.21608/ejm.2020.19131.1124

Keywords

Rhamnolipids, Personal Care Formulation, toxicity, Bio-preservative, <i> Pseudomonas aeruginosa </i>

Authors

First Name

Atef M.

Last Name

Diab

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

Email

atefdiabdaibfeta55@yahoo.com

City

-

Orcid

-

First Name

Sami

Last Name

Ibrahim

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

Email

sami.ibrahim@arma.com.eg

City

-

Orcid

0000-0001-8730-1647

First Name

Hesham M.

Last Name

Abdulla

MiddleName

-

Affiliation

Botany Department, Faculty of Science, Suez Canal University, Ismailia, Egypt

Email

hesham_abdulla@science.suez.edu.eg

City

Ismailia

Orcid

0000-0001-6901-1611

Volume

55

Article Issue

The 14th Conference of Applied Microbiology

Related Issue

14972

Issue Date

2020-06-01

Receive Date

2019-11-05

Publish Date

2020-06-01

Page Start

57

Page End

70

Print ISSN

0022-2704

Online ISSN

2357-0881

Link

https://ejm.journals.ekb.eg/article_98700.html

Detail API

https://ejm.journals.ekb.eg/service?article_code=98700

Order

6

Type

Original Article

Type Code

121

Publication Type

Journal

Publication Title

Egyptian Journal of Microbiology

Publication Link

https://ejm.journals.ekb.eg/

MainTitle

Safe Application and Preservation Efficacy of Low-toxic Rhamnolipids Produced from <i> Ps. aeruginosa </i> for Cosmetics and Personal Care Formulation

Details

Type

Article

Created At

22 Jan 2023