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4108

Assessment of Genistein and Daidzein Production By Some Local Fungal and Bacterial Isolates

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Last updated: 01 Jan 2025

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Abstract

TWENTY-THREE different bacterial and fungal isolates were grown and screened for their capability to transform soy glycosides to their aglycone forms with higher titer of antioxidant activity compared to unfermented soy flour. Most of the bacterial isolates showed higher amounts of daidzein than of genistein, which are the aglycone products of daidzin and genistin. After fermentation of soybean flour using bacterial and fungal isolates, the content of isoflavone aglycones varied from 0.0 to 431.89 μg/g compared to unfermented autoclaved soybean flour. Extracellular β-glucosidase activity was ranged from 1.22 to 11.56 mU/mL and 0.3-534.3 U/mL for bacterial and fungal isolates, respectively, while, bacterial cell-bound β-glucosidase ranged from 44.72 to 128.89 mU/mL. Most of the bacterial isolates more efficiently transformed daidzin and genistin into the aglycones than fungal isolates. Among the tested bacterial isolates, the most potent one was selected, characterized according to the morphological and 16S rDNA sequence analysis and identified as Bacillus licheniformis NRC24.

DOI

10.21608/ejm.2017.1134.1023

Keywords

Daidzein, genistein, β-glucosidase, free radical DPPH-scavenging activity, phylogentic tree, <i> B. licheniformis </i> NRC24

Authors

First Name

Asmaa

Last Name

El-Shazly

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drugs Industries Research Division, National Research Centre, Cairo, Egypt

Email

asmaa.elshazly@yahoo.com

City

Giza

Orcid

-

First Name

Azza

Last Name

Noor El-Deen

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drugs Industries Research Division, National Research Centre, Cairo, Egypt

Email

ranim94@yahoo.com

City

Cairo

Orcid

-

First Name

Nevin

Last Name

Ibrahim

MiddleName

-

Affiliation

Department of Microbiology, Ain Shams University, Cairo, Egypt

Email

nevin_a_ibrahim@yahoo.com

City

-

Orcid

-

First Name

Nayera

Last Name

Abdelwahed

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drugs Industries Research Division, National Research Centre, Cairo, Egypt

Email

niarawahed@yahoo.com

City

-

Orcid

-

First Name

Ahmed

Last Name

El-Beih

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drugs Industries Research Division, National Research Centre, Cairo, Egypt

Email

aae2eg@yahoo.com

City

-

Orcid

-

First Name

Yousseria

Last Name

Shetaia

MiddleName

-

Affiliation

Department of Microbiology, Ain Shams University, Cairo, Egypt

Email

you_shetaia@hotmail.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Farid

MiddleName

-

Affiliation

Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drugs Industries Research Division, National Research Centre, Cairo, Egypt

Email

nrcfarid@yahoo.com

City

-

Orcid

-

Volume

52

Article Issue

1

Related Issue

826

Issue Date

2017-12-01

Receive Date

2017-05-31

Publish Date

2017-12-01

Page Start

49

Page End

61

Print ISSN

0022-2704

Online ISSN

2357-0881

Article File

EJM_Volume 52_Issue 1_Pages 49-61.pdf

PDF . 1.3MB

Link

https://ejm.journals.ekb.eg/article_4108.html

Detail API

https://ejm.journals.ekb.eg/service?article_code=4108

Order

5

Type

Original Article

Type Code

121

Publication Type

Journal

Publication Title

Egyptian Journal of Microbiology

Publication Link

https://ejm.journals.ekb.eg/

MainTitle

Assessment of Genistein and Daidzein Production By Some Local Fungal and Bacterial Isolates

Details

Type

Article

Created At

22 Jan 2023