BIOFILMS production during bacterial urinary tract infections (UTIs), being responsible for persistence and relapses. Bacterial producing biofilms are difficult to be eradicated as they reveled antibiotics resistant phenotype that correlated to provided protections by biofilms. The present study revealed that the gram negative bacteria (G-ve) were the most common uropathogenic bacteria causing UTIs with 64.28% biofilm producing ability especially Escherichia coli and Klebsiella pneumoniae. While, gram positive bacteria (G+ve) represented 23.63% of UTIs with 57.69% biofilm producing ability. The isolated uropathogenic bacteria demonstrated a high and widespread resistance (50% to 95 %) to all used antibiotics except Nitrofurantoin and Imipenem. The most antibiotics resistant uropathogenic isolates were biofilm producers and some of them revealed haemolytic activity. The present study indicated the importance of studying biofilm producing ability of uropahogenic bacteria as it represented 62.72% during UTIs. The anti-biofilm activity of cell free supernatant of Lactobacillus plantarum subsp. plantarum DSMZ 20174 and Lactobacillus acidophilus DSMZ 20079T against biofilms of both G-ve and G+ve uropathogenic bacteria beside their acid and bile salt tolerance gave potentiality for their use during UTIs as a beneficial tool for dissociation or prevention of biofilms and enable antimicrobial drugs from eradication of infections.