IN THE PRESENT study, radioresistant bacterial strains isolated from the dried mixed populations of bacteria of, eye make up preparations were used. The mixed population of bacterial strains from eye shadow, eye mascara, and eye liner, survived the radiation doses of 5-14 kGy, 5-6 kGy, and 4-11 kGy, respectively. Dose survival curves of the radioresistant bacterial strains exhibited the exponential response, except Bacillus cereus which showed non exponential response towards gamma radiation. The D10 values were in the range of 0.7-1.8 kGy, 0.8-1.0 kGy and 0.7-1.4 kGy for the strains from eye shadow, eye mascara, and eye liner, respectively. The sterilization doses for the eye make up products were determined from the dose response curves. The maximum sterilization doses were found to be 16.0, 8.3 and 12.3 kGy for sterilization of the eye makeup products at the same mentioned order.