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Removal of Lead(II) by <i>Saccharomyces cerevisiae </i>AUMC 3875

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Last updated: 22 Jan 2023

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Abstract

THE REMOVAL of lead(II) from artificial aqueous solution using live and dead biomass of Saccharomyces cerevisiae AUMC 3875 was investigated. The minimum inhibitory concentration (MIC) value of S. cerevisiae AUMC 3875 for lead(II) was 600mg/l. Maximum lead(II) uptake capacities were achieved at pH 5.0 and initial metal ion concentration 300mg/l using 3g/l live and dead cells, respectively. Maximum biosorption capacities were reached after 3h and 20min for live and dead cells, respectively. Fourier Transform Infrared spectroscopy (FTIR) results revealed the important role of C=O, ـــ OH, ـــ NH, protein amide II band,  2 PO , mannans, sulphur and sulphur-oxygen compounds in lead(II) uptake. Scanning electron microscopy analysis (SEM) showed that the cell surface morphology and surface area/volume ratio changed greatly after lead(II) uptake. Transmission electron microscopy analysis (TEM) confirmed the involvement of both extracellular adsorption and intracellular penetration through the cell wall. X-ray powder diffraction (XRD) analysis revealed the presence of Pb(SO4), Pb2OSO4 in dead yeast cells and Pb3O2(SO4),Pb2OSO4 in live biomass. Energy dispersive Xray microanalysis (EDAX) confirmed the occurrence of sulphur, oxygen and lead(II) on the cell wall. The removal of lead(II) from storage battery industry wastewater was performed by dead yeast cells efficiently.

DOI

10.21608/ejm.2013.245

Keywords

<i>Saccharomyces cerevisiae, ></i> Lead(II), Biosorption, SEM, TEM, FTIR, EDAX, XRD

Volume

48

Article Issue

1

Related Issue

86

Issue Date

2013-12-01

Receive Date

2012-09-19

Publish Date

2013-12-31

Page Start

1

Page End

21

Print ISSN

0022-2704

Online ISSN

2357-0881

Link

https://ejm.journals.ekb.eg/article_245.html

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https://ejm.journals.ekb.eg/service?article_code=245

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1

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Original Article

Type Code

121

Publication Type

Journal

Publication Title

Egyptian Journal of Microbiology

Publication Link

https://ejm.journals.ekb.eg/

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Article

Created At

22 Jan 2023