Background: The incidence of male reproductive disorders has been increased and may be linked to the environmental natural and synthetic chemicals. These chemicals are known as endocrine disrupting chemicals (EDCs); Bisphenol-A (BPA) is the most common one. Platelet rich plasma (PRP) is an autogenous and economical source of growth factors which nowadays used in the tissue repair.
Aim of the Work: Evaluate the possible therapeutic effect of PRP against BPA induced prostatic lesions in adult albino rats.
Materials and Methods:Thirty five adult male albino rats were divided into 3 main groups.Group I served as control, Group II:the rats were received 50 mg/kg BPA once daily for 4 weeks, Group III: the rats were received BPA as group II then 0.5 ml of PRP subcutaneous injected twice weekly for 4 weeks. After 8 weeks, rats of all groups were subjected to the followings; biochemical analysis of serum testosterone, immunohistochemical studies for androgen and estrogen receptors (AR &ER), light and electron microscope examination.
Results:Serum testosterone levels showed a highly significant decrease in group II when compared to others. Histologically, BPA treated prostate showed abundant collagen fibers with congested blood vessels and inflammatory cellular infiltration while, their acini showed numerous folds with epithelial stratification. Ultrastructurally, the secretory cells had heterochromatic nuclei, dilated endoplasmic reticulum, apical few microvilli and polymorphicsecretory granules with lysosomes. Nearly all acinar cells showed negative nuclear ARs while positive ERs detectionin basal cells. After PRP administration, showed improvement of many of these changes.
Conclusion: Exposure to BPA at low dose induced deleterious effects on the histological structure of the prostate and disturbed reproductive hormones. PRP showed significant signs of restoration of these changes.