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145750

Biochemical, Histological, and Immunohistochemical Changes Associated with Alcl3- Induced Hepatic Injury in Rats: Protective Effects of L-carnitine

Article

Last updated: 22 Jan 2023

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Abstract

Background: There has been a great interest in the toxicity of Aluminum (Al) due to its environmental wide distribution and every day usage. The key mechanisms of Al -induced toxicity in the liver are recognized as reactive oxygen species (ROS), development of free radicals, oxidative stress, and lipid peroxidation. L-carnitine is a conditionally important amino-acid (4-N-trimethylammonium-3-hydroxybutyric acid). By L-carnitine, long-chain fatty acids are taken into the mitochondria contributing to the metabolism of cellular energy. Also, it can enhance the antioxidant status by accelerating the free radicals removal from cells.
Objective: The purpose of this report was to determine the possible detrimental impact of Alcl3 and to evaluate for the first time the possible potential hepato-protective effect of exogenous L-carnitine supplementation in ameliorating these possible deteriorations.
Materials and Methods: Thirty two rats were subdivided into equal four groups, Group I: Control rats, Group II: Rats treated with L-carnitine at a dose of 200 mg/kg. b.wt., Group III: Rats treated with Alcl3 at a dose of 100 mg/kg. b.wt., and Group IV: Rats treated with L-carnitine and Alcl3 (200 and 100 mg/kg. b.wt., respectively). All procedures of given materials to animals were orally once daily for one month.
Results: The current investigation showed that Alcl3 ingestion caused an obvious hepatic deterioration evidenced by increased liver enzymes level and imbalance in oxidant/antioxidant status. This was accompanied by histological changes and increased caspase-3 immunoreactivity. These effects were significantly improved by L-carnitine supplementation.
Conclusion: These findings suggested that L-carnitine may have a protective effect against hepatic damage sustained by Alcl3 through its antioxidative property and its inhibitory effect on apoptosis.

DOI

10.21608/ejh.2021.52300.1395

Keywords

AlCl3, biochemistry, histology and immunohistochemistry, L-carnitine

Authors

First Name

Basant

Last Name

Mohamed

MiddleName

-

Affiliation

Zoology Department, Faculty of science

Email

chem.b.mahmoud@gmail.com

City

cairo

Orcid

-

First Name

Nagui

Last Name

Fares

MiddleName

Hassan

Affiliation

Zoology department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

nagui_hf@yahoo.com

City

Cairo

Orcid

-

First Name

Neveen

Last Name

Ashaat

MiddleName

Aseem

Affiliation

Faculty of Women for Arts, Science and Education, Ain Shams University

Email

neveen_asem@yahoo.com

City

cairo

Orcid

-

First Name

Faten

Last Name

Abozeid

MiddleName

-

Affiliation

Zoology department, Faculty of Science, Ain Shams University, Cairo, Egypt

Email

faten.sabra@yahoo.com

City

-

Orcid

0000-0002-3888-7503

Volume

45

Article Issue

1

Related Issue

33206

Issue Date

2022-03-01

Receive Date

2021-01-06

Publish Date

2022-03-01

Page Start

90

Page End

100

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_145750.html

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https://ejh.journals.ekb.eg/service?article_code=145750

Order

6

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

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Details

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Article

Created At

22 Jan 2023