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101080

A Comparative Study on the Protective Role of Silymarin and Coenzyme-Q10 on the Cerebellar Cortex of Experimentally Induced Atherosclerosis in Adult Male Albino Rats: A Histological, Immunohistochemical and Biochemical Study

Article

Last updated: 22 Jan 2023

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Abstract

Background: Atherosclerosis is known as a serious cardiovascular condition that causes human mortality worldwide. Atherosclerosis is a significant progressive condition of inflammation. Atherosclerosis of the cerebellar arteries affects voice, balance and fine motor control. Coenzyme-Q10 vitamin soluble in fat that used for heart failure and angina treatment protocol. It has the potential to influence cellular bioenergetics, and counteract free radical damage. This has proven success in treating many neurodegenerative diseases. Silymarin is a flavonoid extract of the herb Silybum marianum. It has been reported as the gold medicine in liver disorders and evaluated recently as a neuroprotective.
Objective: Comparison of potential neuroprotective effects of Co Q10 and Silymarin on adult male albino rats cerebellar cortex suffering from atherosclerosis.
Materials and Methods: Sixty adult male albino rats were divided randomly into equal four classes: Group I (control group) subdivided into three subgroups: Subgroup IA: provided ordinary diet. Subgroup IB (Co enzyme Q10 treated): obtained ordinary diet and co-enzyme Q10 Subgroup IC (Silymarin treated): obtained ordinary diet and Silymarin. Group II (atherosclerotic group), Group III (atherosclerotic Co Q10 treated group) and Group IV (atherosclerotic Silymarin treated group). After three months, lipid profile was assessed in blood samples taken from all classes. Cerebellar cortices were processed for histological, biochemical, immunohistochemical and electron microscopic studies.
Results: Atherosclerotic rats showed loss and disruption of Purkinje cell layer with pyknotic nuclei, dilated cisternae of rough endoplasmic reticulum, prominent perineural spaces and spongiosis of granular and molecular layers. Significant increase in GFAP, Cox-2 immunoreactivity was also found. However, concomitant administration of Co Q10 or Silymarin had ameliorating effect on these changes.
Conclusion: Co Q10 or Silymarin supplementation had a neurological protective role in atherosclerosis. Silymarin may be the ideal neuroprotective agent and new approach for reduction of many neurological diseases in high risk population.

DOI

10.21608/ejh.2020.28009.1276

Keywords

Atherosclerosis, Coenzyme-Q10, Purkinje cells, Silymarin, Spongiosis

Authors

First Name

nadia

Last Name

badawy khair

MiddleName

said

Affiliation

histology department.faculty of medicine,menuofiya university

Email

amarsmile2007@yahoo.com

City

shebin elkom

Orcid

-

First Name

seham

Last Name

mohammed

MiddleName

ahmed

Affiliation

histology an cell biology -faculty f medicine menoufia university

Email

dr.sehamhist@gmail.com

City

ashmun

Orcid

0000-0003-1183-4353

Volume

44

Article Issue

2

Related Issue

27775

Issue Date

2021-06-01

Receive Date

2020-05-26

Publish Date

2021-06-01

Page Start

322

Page End

338

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_101080.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=101080

Order

3

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

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Details

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Article

Created At

22 Jan 2023