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58545

The Possible Therapeutic Role of Ginger Extract in the Effect of Chronic Aluminum Toxicity on Rat Periodontium (Histological and Immunohistochemical Study)

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Last updated: 22 Jan 2023

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Abstract

Background: Chronic aluminum intoxication enhanced the risk for different body tissues in human and animals. Ginger administration may minimize the harmful effects of metal ions toxicity.
Objective: This study has attempted to assess and compare the effects of chronic aluminum intoxication and concomitant ginger treatment on different structures of rat periodontium.
Methodology: 21 adult male albino rats were divided into three equal groups: For three months; groupI received sterile 0.9% saline/day orally, groups II and III received (20mg/kg/day) aluminum chloride orally in drinking water; then, only groupIII received (150mg/kg/day) ginger oil extract orally for 4 weeks. Some of dissected mandibular halves were used for bone mineral density (BMD) measure. Other halves were decalcified and processed for H&E and immunohistochemical (using anti-CD68+ and anti-osteopontin antibodies) examination.
Results: Histopathologically, groupII showed apparently decreased periodontal fibers density, wide degenerative areas and marked inflammatory infiltrates. Likewise, CD68+ was significantly expressed in giant cells in periodontal ligament (PDL) and at resorption surfaces of cementum and bone in groupII. These changes were improved in groupIII with a significant decrease of CD68+ positive cells in PDL. Cementum and bone of groupII presented significant destructive changes that were mostly restored in groupIII. Comparing to groupI, osteopontin expression in groupII significantly increased in acellular cementum(AC)>cellular cementum(CC)>bone> PDL. In comparison, this reactivity was significantly increased in bone>CC>AC in groupIII while osteopontin expression in PDL was mild but with significant increase comparing to groupI. BMD of alveolar bone was highest in groupI>groupIII>groupII with a significant differences.
Conclusion: We concluded that detrimental alterations associated with chronic Al toxicity were markedly detected in PDL>bone>CC>AC that could result in serious clinical outcomes. Ginger extract greatly ameliorated these changes in bone>CC>AC>PDL confirming its strong curative potency against aluminum associated oxidative damage thus it is indicated for use in further new therapeutic approaches

DOI

10.21608/ejh.2019.18936.1192

Keywords

aluminium, CD68+, Ginger, Osteopontin, periodontium

Authors

First Name

Fatma

Last Name

Saad

MiddleName

Adel

Affiliation

PhD, Lecturer, Oral biology department, Faculty of Dentistry, Future University in Egypt,

Email

femy76@hotmail.com

City

Cairo

Orcid

0000-0001-6209-8654

Volume

43

Article Issue

2

Related Issue

17203

Issue Date

2020-06-01

Receive Date

2019-10-31

Publish Date

2020-06-01

Page Start

630

Page End

644

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_58545.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=58545

Order

20

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

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Article

Created At

22 Jan 2023