Beta
36069

Effect of Vitamin D on the Spleen of Adult Male Rats Fed on Diet with High Fat: A Histological and Immunohistochemical Study

Article

Last updated: 01 Jan 2025

Subjects

-

Tags

-

Abstract

Background: High fat diet (HFD) is the cause of obesity prevalence which is a serious health problem. Vitamin D is considered a very important modulator in cellular inflammation and immune systems.
Aim of the work: To demonstrate the histological changes in the spleen of the adult male rats fed with HFD and to evaluate vitamin D role in counteracting the harmful effects of HFD on the spleen.
Materials and Methods: A total number of 36 male adult albino rats which were classified equally and randomly into three groups as follows; Control group where rats were administered the control diet for a period of 10 weeks, HFD fed group which was administered HFD for 10 weeks and HFD + vitamin D group which was administered HFD concomitantly with vitamin D orally daily for 10 weeks at a dose of 1 ug (40 IU)/kg. Samples from the spleen were prepared for histological and immunohistochemical study.
Results: The group received HFD revealed marked histological alterations with lymphatic follicles proliferation. Most splenic cells showed degenerative changes. Deposition of hemosidrin pigments, markedly dilated blood sinusoids and blood extravasation were observed. CD86 had a strong cytoplasmic expression in dendritic cells. Moreover, cytoplasmic immune reaction for iNOS and caspase-3 in the white and red pulps of the spleen was intense. Vitamin D co-adminstration greatly preserved the histological and immunohistochemical structure of the spleen.
Conclusion: This study suggests that vitamin D has a role in splenic protection and can attenuate the deleterious effects commonly detected in the spleen and immune system in case of obesity induced by HFD.

DOI

10.21608/ejh.2019.13030.1120

Keywords

CD86, Obesity, spleen, Vitamin D

Authors

First Name

Heba

Last Name

Mohamed

MiddleName

Kamal

Affiliation

Department of Human Anatomy and emberyology- Faculty of Medicine- Assiut University- Egypt

Email

hebaelgamae73@yahoo.com

City

Assiut

Orcid

-

Volume

42

Article Issue

4

Related Issue

10604

Issue Date

2019-12-01

Receive Date

2019-05-20

Publish Date

2019-12-01

Page Start

1,001

Page End

1,017

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_36069.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=36069

Order

16

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

Effect of Vitamin D on the Spleen of Adult Male Rats Fed on Diet with High Fat: A Histological and Immunohistochemical Study

Details

Type

Article

Created At

22 Jan 2023