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Histological Study of the Effect of Pristine Versus Functionalized Polyethylene Glycol - Multiwalled Carbon Nanotubes on the Lung Alveoli of the Adult Male Albino Rats

Article

Last updated: 01 Jan 2025

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Abstract

Background: Carbon nanotubes are used in a variety of applications. However, their toxicity on different body tissues has an impact on human health. Therefore, functionalization emerged as a tool to reduce CNTs toxicity and hence expand their applications.
Aim: The goal of this study was to clarify the impact of pristine MW CNTs on lung alveoli of the adult male albino rat and the possibility of amelioration of this effect by functionalized PEG-MWCNTs.
Materials and Methods:60 adult male rats were categorized among 3 groups. Group I: control group, group II: 20 rats received an intratracheal instillation of a single dose of 1 mg/kg of pristine MWCNTs, then subdivided into two equal subgroups (each 10 rats) and were sacrificed after 3 days and 45 days respectively, group III: 20 rats were received an intratracheal instillation of a single dose of 1 mg/kg of PEG-MWCNTs and then subdivided and sacrificed as in group II. At the end of the experiment, the lungs were harvested for histological examination. The thickness of the interalveolar septum and the collagen area were morphometrically evaluated and statistically analyzed.
Results: Pristine MWCNTs caused variable degree of histological changes that were more evident after 45 days. These changes manifested by collapsed alveoli alternating with wide ones, thickening of the interalveolar septa, deposition of collagen, cellular and nuclear changes suggestive of pulmonary toxicity and accumulation of particulate laden macrophages. Morphometric and statistical studies demonstrated significant increase in the thickness of the interalveolar septa as compared to control group. Ultrastructurally, type II pneumocytes showed degenerative changes and formed the predominant cell lining. The changes were less evident by instillation of functionalized PEG-MWCNTs with more or less preservation of the normal alveolar architecture.
Conclusion: Pristine MWCNTs have a toxic effect on the lung tissue which could be reduced by functionalized PEG-MWCNTs.

DOI

10.21608/ejh.2019.12809.1119

Keywords

carbon nanotubes, histology, polyethylene glycol, pristine

Authors

First Name

Safinaz

Last Name

Safwat

MiddleName

Hussein

Affiliation

Faculty of Medicine Alexandria University, Histology & cell Biology department

Email

safinaz_safwat@yahoo.com

City

Alexandria

Orcid

-

First Name

Iman

Last Name

Nabil

MiddleName

-

Affiliation

Alexandria faculty of medicine, histology and cell biology departement

Email

emannabil4@gmail.com

City

Alexandria

Orcid

-

First Name

Wahid

Last Name

Stefanos

MiddleName

Moufid

Affiliation

Alexandria Faculty of Medicine, Histology & cell biology department

Email

wahidstephanos@hotmail.com

City

Alexandria

Orcid

-

First Name

Marwa

Last Name

Ahmed

MiddleName

Magdi

Affiliation

Alexandria Faculty of Medicine, Histology & cell biology department

Email

dr_mmak55@yahoo.com

City

Alexandria

Orcid

-

Volume

42

Article Issue

4

Related Issue

10604

Issue Date

2019-12-01

Receive Date

2019-05-15

Publish Date

2019-12-01

Page Start

928

Page End

955

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_34767.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=34767

Order

13

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

Histological Study of the Effect of Pristine Versus Functionalized Polyethylene Glycol - Multiwalled Carbon Nanotubes on the Lung Alveoli of the Adult Male Albino Rats

Details

Type

Article

Created At

22 Jan 2023