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62541

Effect of Hematopoietic Stem Cells and Platelet-Rich Plasma on the Healing of Experimental Skin Burned Tissues: A Comparative Study in Adult Male Mice

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Last updated: 01 Jan 2025

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Abstract

Background: Unfortunately burns are a common problem, leading to scarring or death. Platelet rich plasma (PRP) is a well known method that could be used as topical application for burn either second or third degree; it harbors many growth factors that can accelerate the healing.  This Study Aimed: To analyze the use of PRP in deep second-degree burn, in comparison with the hematopoietic stem cells (CD34+) in mice.  Materials and Methods: Seventy male adult mice were divided into four groups (control group, burn only, burn treated with CD34+ cells (injected once intradermal with 0.3–0.4 × 106 /kg and burn treated with PRP (1 ml injected once intradermal) at the edge of burn after 24hs from its induction. All burned groups were exposed to thermal burn. On day 9 and 19, the animals were sacrificed and skin biopsies were taken for H&E and Masson's trichrome staining. Assessment of angiogenesis and quantification study for matrix metalloproteinase 13 (MMP13) was done.  Results: Wounds treated with PRP revealed fast wound closure in comparison with burned only group. Significant decrease in collagen fibers and increase in MMP13 deposition, as well as angiogenic markers (Angio1 and 2 and VEGF) expression was observed. However CD34+ cells treated group showed highly significant improvement in previously mentioned markers. Histopathological changes of burned skin improved in all treated groups after 19 days particularly in CD34 + cells treated group.  Conclusion: These results indicated that CD34+ cells treatment could exert beneficial effects on healing process more than that of PRP in second degree burns.

DOI

10.21608/ejh.2019.8023.1083

Keywords

Angiogenesis, collagen, hematopoietic stem cell, MMP13, platelet-rich plasma

Authors

First Name

Heba

Last Name

Saad Eldien

MiddleName

Mohamed

Affiliation

Histology and cell biology - Faculty of Medicine- Assiut University

Email

hebasaadeldien2015@yahoo.com

City

Assiut

Orcid

-

First Name

Nashwa

Last Name

Mostafa

MiddleName

A.

Affiliation

Department of Histology And Cell biology , College of Medicine, Assiut University,

Email

nashwa20012002us@aun.edu.eg

City

-

Orcid

-

First Name

Ola

Last Name

Abd ElTawab

MiddleName

-

Affiliation

Department of Histology And Cell biology , College of Medicine, Assiut University

Email

-

City

-

Orcid

-

First Name

Hussein

Last Name

Hassan

MiddleName

-

Affiliation

Department of Histology And Cell biology , College of Medicine, Assiut University,

Email

-

City

-

Orcid

-

First Name

Tarek

Last Name

Abd Elhamid

MiddleName

Hamdy

Affiliation

Department of Histology And Cell biology , College of Medicine, Assiut University,

Email

-

City

-

Orcid

-

First Name

Nesreen

Last Name

Abd Elhaliem

MiddleName

G.

Affiliation

Department of Histology, College of Medicine, Sohag University

Email

-

City

-

Orcid

-

First Name

Eman

Last Name

El Shall

MiddleName

-

Affiliation

Department of Anatomy, Alazhar University

Email

-

City

-

Orcid

-

First Name

Douaa

Last Name

Sayed

MiddleName

M.

Affiliation

Department of Clinical pathology, South Egypt Cancer Institute

Email

-

City

-

Orcid

-

First Name

Amany

Last Name

Mohamed

MiddleName

Osama

Affiliation

Department of medical biochemistry, College of Medicine, Assiut

Email

-

City

-

Orcid

-

First Name

Nahed

Last Name

Mohamed

MiddleName

A.

Affiliation

Department of medical biochemistry, College of Medicine, Assiut

Email

-

City

-

Orcid

-

First Name

Ahlam

Last Name

Abdalla

MiddleName

M.

Affiliation

Medical biochemistry College of Medicine, Minia University

Email

-

City

-

Orcid

-

First Name

Salama

Last Name

Abdelraheim

MiddleName

-

Affiliation

Medical Biochemistry College of Medicine, Minia University

Email

-

City

-

Orcid

-

First Name

Nasser

Last Name

Abou Khalil

MiddleName

Sayed

Affiliation

Department of Medical physiology, College of Medicine, Assiut University

Email

-

City

-

Orcid

-

First Name

Ahmed

Last Name

Kamal

MiddleName

-

Affiliation

Department of Plastic surgery College of Medicine, Assiut University

Email

-

City

-

Orcid

-

Volume

42

Article Issue

3

Related Issue

9427

Issue Date

2019-09-01

Receive Date

2019-03-14

Publish Date

2019-09-01

Page Start

740

Page End

754

Print ISSN

1110-0559

Online ISSN

2090-2417

Article File

EJH_Volume 42_Issue 3_Pages 740-754.pdf

PDF . 3.7MB

Link

https://ejh.journals.ekb.eg/article_62541.html

Detail API

https://ejh.journals.ekb.eg/service?article_code=62541

Order

18

Type

Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

MainTitle

Effect of Hematopoietic Stem Cells and Platelet-Rich Plasma on the Healing of Experimental Skin Burned Tissues: A Comparative Study in Adult Male Mice

Details

Type

Article

Created At

22 Jan 2023