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15905

Histological study on the possible protective effect of curcumin on potassium dichromate induced hypothyroidism in adult male albino rats

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Last updated: 22 Jan 2023

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Abstract

Introduction: Potassium dichromate, a widely used heavy metal in several industries induces hypofunction and tissue insult of the thyroid gland via oxidative stress. Curcumin; is a natural commonly used spice has a strong anti-inflammatory and antioxidant properties.
Aim of the work: To investigate the possible protective effect of curcumin on the hypothyroidism induced by potassium dichromate in albino rats.
Materials and Methods: Thirty- five adult male albino rats were divided into five groups, 15 rats in group I (control) and 5 rats in each of group II, III, IV and V. Group II (curcumin group) received curcumin orally (100 mg /kg/bw) daily for 4 weeks. Group III (potassium dichromate induced hypothyroidism) received i.p injection of potassium dichromate (2 mg/kg/bw) daily for 2 weeks. Group IV (recovery group) received potassium dichromate as group III then left untreated for another 2 weeks. Group V(curcumin and potassium dichromate group) received curcumin concomitant with potassium dichromate as in groups II and III, respectively daily for 2 weeks and only curcumin was continued for another 2 weeks. T3, T4 and TSH were assessed. Thyroid sections were subjected to toluidine blue, H&E, PAS and PCNA immunohistochemical stains. Morphometric and statistical studies were done.
Results: Thyroid tissue insult and hypofunction with significantly decreased T3, T4 and increased TSH were detected in group III. Additionally, there was a significant increase in the mean values of follicular cell height, follicular diameter, mean number of PCNA positive nuclei and a significant decrease in the mean value of area percent of colloid versus group I, II and V with non-significant differences versus group IV. In group V, there was an obvious serological and histological improvement compared to group III and IV.
Conclusion: Curcumin had protective effect against hypothyroidism and thyroid tissue damage induced by potassium dichromate.

DOI

10.21608/EJH.2018.13844

Keywords

Curcumin, hypothyroidism, PCNA, Potassium Dichromate, rat, THYROID

Authors

First Name

Gihan

Last Name

Aboul-Fotouh

MiddleName

Ibrahim

Affiliation

Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt

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First Name

Rahma

Last Name

Abou El-Nour

MiddleName

Kamal El- Din

Affiliation

Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt

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City

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Orcid

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First Name

Eman

Last Name

Farag

MiddleName

-

Affiliation

Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt

Email

dr.emanabas@yahoo.com

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-

Orcid

-

First Name

Wafaa

Last Name

Boughdady

MiddleName

Abd El-Azeem Abdou

Affiliation

Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt

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Orcid

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Volume

41

Article Issue

2

Related Issue

3418

Issue Date

2018-06-01

Receive Date

2018-10-04

Publish Date

2018-06-01

Page Start

220

Page End

235

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_15905.html

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https://ejh.journals.ekb.eg/service?article_code=15905

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8

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Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

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Article

Created At

22 Jan 2023