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The Possible Protective Role of Ginger Extract Versus Vitamin E Against Simvastatin-Induced Skeletal Myotoxicity in Adult Male Albino Rats: Histological, Physiological and Biochemical Study

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Last updated: 22 Jan 2023

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Abstract

Introduction: Statins are group of drugs used to reduce total and low density lipoprotein (LDL)-cholesterol level and to reduce the morbidity and mortality of cardiovascular diseases. Meanwhile, induced skeletal muscle- specific mitochondrial impairment, oxidative stress and myotoxicity are serious side effects . Vitamin E and ginger extract are well known potent antioxidants.
Aim: To study the possible protective effect of ginger extract versus vitamin E against simvastatin- induced skeletal muscle histological and the associated biophysiological changes.
Materials and Methods: Forty adult male albino rats were randomly divided into four equal groups (10 rats, each).; Group 1: was the control rats. Group 2: received 0.54 mg/kg/day simvastatin orally for 8 weeks. Group 3: received concomitant treatment of simvastatin and 30 mg/kg/day vitamin E orally for 8 weeks. Group 4 received concomitant treatment of simvastatin and 500mg/kg/day ginger extract orally for 8 weeks. After sacrifice, specimens were taken from the belly of the quadriceps femoris muscles of all animal groups and processed for light and electron microscopy. Biochemical tests and statistical analysis were done.
Results: Group 2 showed focal areas of muscle fiber loss, mononuclear cellular infiltration and variable staining density. Ultra structurally, myofibrillar degeneration and accumulation of numerous giant infrequently damaged mitochondria were observed. The skeletal muscle fibers of animals from group 3 and group 4, both were markedly improved. Group 4 revealed obviously normal mitochondria.
Conclusion: Administration of simvastatin for 8 weeks induced histological, physiological and biochemical skeletal myotoxic effects. These effects were greatly ameliorated by concomitant administration vitamin E or ginger extract. Ginger extract was more effective.

DOI

10.21608/EJH.2017.4074

Keywords

Ginger extract, mitochondrial biogenesis, myotoxicity, oxidative stress index, Simvastatin, Ultrastructure, Vitamin E

Authors

First Name

Kawther

Last Name

Abdel Hamid

MiddleName

M.

Affiliation

Histology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

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Orcid

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First Name

Asmaa

Last Name

Abdel Mola

MiddleName

Fathi

Affiliation

Histology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

asmaanoorahmed@yahoo.com

City

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Orcid

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First Name

Fatma

Last Name

Meligy

MiddleName

Y.

Affiliation

Histology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

Email

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City

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Orcid

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First Name

Eman

Last Name

Abd Allah

MiddleName

S. H.

Affiliation

Medical Physiology Department, Faculty of Medicine, Assiut University, Assiut, Egypt

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Orcid

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Volume

40

Article Issue

2

Related Issue

712

Issue Date

2017-06-01

Receive Date

2016-08-30

Publish Date

2017-06-01

Page Start

156

Page End

168

Print ISSN

1110-0559

Online ISSN

2090-2417

Link

https://ejh.journals.ekb.eg/article_4119.html

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https://ejh.journals.ekb.eg/service?article_code=4119

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3

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Original Article

Type Code

119

Publication Type

Journal

Publication Title

Egyptian Journal of Histology

Publication Link

https://ejh.journals.ekb.eg/

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Article

Created At

22 Jan 2023