A field experiment was conducted in a private farm at Tour Sinai, South Sinai, during two consecutive winter seasons of 2019/2020 and 2020/2021 to study the influence of bio-fertilization treatments (with and without bio-fertilizer) and bio-stimulant treatments, which contain ‘marine algae extract, humic acid and some micro elements', on vegetative and yield traits as well as chemical constituents of black cumin (Nigella sativa L.) plants. The bio-stimulants were applied as control, 5 ml/l and 10 ml/l as foliar spray, 5 ml/l and 10 ml/l as soil drench. From the results it was observed that, all growth traits, yield components, oil production as well as chemical components in seeds were affected significantly by the bio-fertilizer treatment. Also, all bio-stimulant treatments showed significant differences in this regard in comparison with control treatment. The superior interaction treatment in this respect was that the combination between the bio-fertilizer and bio-stimulant at 10 ml/l as foliar spraying, which recorded a significant increase and the highest values in this concern. Also, scored the major components of the essential oil which were lauric acid, myristic acid, palmitic acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachidic acid and eicosanoid acid, respectively