Lysinibacillus macroides is a plant growth promoting bacteria (PGPB) and phosphate solubilizing bacteria (PSB). Nano-phosphozinc is a nano-compound used to increase yield of grown plants (Spinach) and decrease the added doses of mineral fertilizers. When compared to controls, the results showed that using treatment with 50 ppm of nano-phosphozinc + L. macroides increased fresh weight by approximately 169.08 and 77.47% for fresh matters, respectively, and increased dry weight by approximately 273.3 and 84.27% for the first and second cuts, respectively. L. macroides produced organic acids, phytohormones, acidic and alkaline phosphatase enzymes and it had a phosphate solubilizing activity. L. macroides could survive nano-phosphozinc concentrations of up to 15 ppm. It is important to do more studies on the effects of nanomaterials on a long time to know its accumulation on human body, soil, plant, micro-organism activities and environment.