Heat stress is one of the major abiotic stresses affecting potato growth and productivity in semi-arid and growing zones. Using new biotechnology approaches and application of nanomaterials could improve the productivityand profitability of potato.Silicon nanoparticles(SiO₂NPs)responsible for improvements lead finally to the enhancement of plant growth and productivity under salinity, drought, and heavy metal stresses.In this study, the effectiveness of SiO₂ NPs at 5, 10 and 20 ppm under different levels of heat of 25, 35 and 45°C on growth and biochemical parameters was in vitrostudied, for four potato cultivars.Results referred to the superiority of Agria, which was positively and significantly affected by SiO₂NP treatments followed by Hermes, and Cara. On the other hand, there was no significant effect of SiO₂NPs on Diamond cv, under different heat levels. At 45°C, Agria cv. produced the highest value of microtubers weight and length at 0, 5, 10 and 20 ppm of SiO₂ NPs, followed by Diamond with SiO₂NPs of 5 and 20 ppm. The heighest vegetative characteristics of Agria were 14.67 cm for shoot length, 7.00 cm for shoots/explant, 5 cm for root length, and 10.33 for roots number/ explant, at 35°C with 10 ppm SiO₂NPs. About harvesting, for the same cultivar, microtubersweight was 0.89, 0.79 and 0.93 g at 45°C, without significant differences among SiO₂NPs.Microtubers number was 5.33 by 5 ppm at 25°C. Microtubers length was 1.43 cm with 5 and10 ppm SiO₂NPs at 45°C.  For microtubers diameter, there was no significant effect among SiO₂NPs at all heat levels. Also, glutathione higher value was obtained by 5 ppm at 45^oC. Concerning lipid peroxidation level, the lowest level of the toxic product malondialdehyde (MDA) was obtained by 0 ppm, increased in other treatments at 25^oC. At 35^oC, the minimum level of MDA was recorded by 20 ppm and in general the highest temperature applied (45^oC) increased MDA accumulation.