Asparagus aphyllusis grown in Egypt naturally as a rare plant.It is utilized in conventional medicine to treat various liver diseases and asanti-cancer. Shoot tips have been cultured on Murashige and Skoog (MS) mediumsupplemented with 1.0 mg/l kinetin (kin) and6-benzyl adenine (BA) combined with different concentrations of α-naphthalene acetic acid (NAA) for the multiplication of shoots.The shoot of 5 cm has been cultured on 1/2 MS medium supplemented with various concentrations of indole-3-butyric acid (IBA) or full MS medium with 1.0 mg/l kin and two concentrations of NAA for rooting. The top results of multiplication were obtained with 1.0 mg/l BA and 0.2 mg/l NAA and gave 12 shoots per explant and the maximum mean length of shoot was 6.60 cm with 1.0 mg/l BAand 0.4 mg/lNAA.The highest percentage of rooting was 20% after three months of culture on half strength MS medium with IBA at 1.5 mg/l and 2.0 mg/l. The mean number of roots per explantwas 1.6 roots. The mean length of root was 4.0 cmat a concentration of2.0 mg/l IBA.Percentage of survival after acclimatization was about 65% after two months. The highest fresh weight of callus was  14.03 g with friable texture and observed on MS medium supplemented with 2.0 mg/l NAA plus 1.0 mg/l 2.4- D  with 0.5 mg/l BA. The total saponin that contains the highest value of 5.85 mg/g fresh weight was observed from the mother plant.The results of the present study would be applicable for cost-effective large-scale micropropagation of Asparagusaphyllusand improvement.