Wheat is one of the most important dietary crops and is a staple food in many countries. Several pathogens attack wheat in different growth stages, stripe rust is considered one of an important disease appears and causes damage by growing in the leaves and producing spores. In this study, the expression levels of four genes from myeloblastosis (MYB) Transcription factor family under compatible (CYR31) and incompatible (CYR23) strains of stripe rust were tested. The results showed TaMYB1transcript levels with CYR31 were induced at 12 hpi and 48 hpi compared with the control which were about 1.5-fold and 3.8-fold, respectively.While, the transcript levels with CYR23 were reduced with all incubated time, except 12 hpi and 48 hpi (about 2.3-fold - 1.7-fold, respectively). In addition, the transcript levels were significantly reduced at 72 hpi with both strains. Moreover, the same results were observed with TaMYB7under the same conditions. The expression levels were significantly induced at 12 hpi and 48 hpi, which were about 1.78-fold and 4-fold, respectively. But, at 24 hpi, the expression pattern of TaMYB7was induced with CYR23.TaMYB2transcript levels were reduced at all time points with both strains, except 12 hpi with CYR23.Furthermore,theTaMYB30transcript level was induced (3.4-fold) at 12 hpi with CYR23 compared with control. On another hand, after 12 hpi, the expression levels of TaMYB30were reduced. Altogether,it can be concluded that TaMYB1,TaMYB2, TaMYB7and TaMYB30 may be played roles during the interaction between wheat and stripe rust pathogen in different stages.