A survey for plant parasitic nematode (PPN) fauna in Sinai Peninsula, Egypt was carried out. A total of 607 soil and root samples was collected, at growth seasons during the period from 2016 to 2017 at both governorates; North Sinai (in which few surveys were accomplished) and South Sinai for the first time. The nematode survey was based on collecting soil and root samples around the roots. In the two governorates, 13 genera in 9 families, belonging to 3 orders of phylum Nematoda were observed.These orders were Dorylaimida, Triplonchida and Tylenchida. Three genera viz., Meloidogyne, Rotylenchulus andTylenchorhynchus belonging to order Tylenchida were found to represent the highest plentiful numbers (18891, 3528 and 3316, respectively) in Sinai Peninsula. Meloidogynewas the most abundant and dominant genus in all surveyed districts. Nematodes in North Sinai were more diverse than South Sinai, since they possessed 3 orders, 8 families and 13 genera (Belonolaimus, Criconema, Criconemoides, Helicotylenchus, Hoplolaimus, Meloidogyne,Pratylenchus, Rotylenchulus, Tetylenchus, Trichodorus, Tylenchorhynchus, Tylenchusand Xiphinema) versus 2 orders, 5 families and 6 genera viz., Criconema, Meloidogyne, Pratylenchus, Rotylenchulus, Trichodorus and Tylenchorhynchusin South Sinai. Species diversity peaked in Rafah district, whereas in South Sinai, peak was recorded in Ras-Suder district. Reviewing the previous investigations carried out in Sinai for recording plant parasitic nematode, it was found that the last 6 genera recovered from South Sinai were the first records, based on the present survey is considered the first nematological study carried out in this governorate.Also, this study willpay the growers attention, to control infestation by PPN.