Two field experimentswere done to assess the effect of seed inoculation with single or dual plant growth promoting rhizobacterial (PGPR) strains (Azotobacter chrococcumand Bacillus subtilis) and three levels of NPK fertilization (50, 75 and 100%) on growth and productivity of barley (Hordeum vulgare) plants growing under saline condition at El-Monira village, El-Kharga Oasis, New Valley, during the two successive seasons 2016 and 2017. Spilt plot design in four replications was used, where NPK fertilization treatments occupied the main plots and biofertilization in the sub-plot. Analysis of variance revealed significant effect of biofertilizer application along with NPK fertilizer levels on all measured growth parameters (plant height, branching, spikes number, fresh and dry weight), soil biological activity (total microbial count, dehydrogenase activity and antioxidant capacity of soil), available nitrogen and phosphorus in soil, uptake of nitrogen and phosphorus, protein content of parley plants and yield and its related characters (spikes/m²numbers, 1000-grains weight and grain yield). Maximum traits were obtained by plants that were treated with 75% of NPK fertilizer and mixed cultures of A. chrococcumand B. subtilis. Thus, it could be concluded that the use of 75% chemical fertilizer along with co-inoculation with A. chrococcumand B. subtilis could promote the growth, productivity and disease resistance in barley under saline condition.