Screening of 51 tomato inbreed lines, obtained from Tomato Breeding Program of Desert Research Center (DRC),was carried out in two different times varied in temperature, early winter and summer during 2018-2019, to evaluate the reaction of these genotypes towards infection with root-gall nematode (RGN) Meloidogyne incognita. The response of these inbreed lines was varied between resistance (R) and highly susceptible (HS) based on galls number (G) and egg masses index (E.M.I.), also egg production was a dependable factor. Two genotypes viz., SA174-7 and SY174-1found to be R according to G and E.M. formed 2 galls and 1 egg mass and 12 galls and 1 egg mass, respectively. The moderately resistant genotypes were SK174-4, SW174-4 and SY174-8-3, while the three genotypes SC174-7, SD174-4-1 and SY174-2-2 found to be moderately susceptible. About 14% of tested genotype were susceptible, while the rest inbreed lines (70%) were HS.It was clearly noticed that temperature (summer and winter trials) has an impact on nematode penetration and reproduction, but its effect in genotype resistance ranking was absent in a few cases. Finally, data obtained from this screening open the way to further evaluations and detection of the genetic diversity of the most resistance genotypes for improving new inbreed lines bearing more desirable characters, in particular high yield with resistance to RGN, and try to identify the genes that conferring resistance to transfer it to susceptible genotypes.