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142476

GENOME-WIDE CHARACTERIZATION OF DROUGHT-RESPONSIVE GENES ACCELERATES FABA BEAN BREEDING PROGRAM

Article

Last updated: 01 Jan 2025

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Subjects

-

Tags

Plants

Abstract

Drought is an influential environmental stress that limits faba bean growth and development, leading to a substantial reduction in their productivity in both arid and semi-arid region of the world. However, the responses of faba bean to drought have not been illustrated very well at molecular level. Six cultivars of faba beans and their fifteen hybrids in F2­were used to investigate the transcriptome, proteome, and metabolome changes. In the present study, two-weeks-old seedlings were deficit by seven days drought stress, whereas control seedlings were watered regularly. The results indicated that water deficiency negatively influences substantial physiological and metabolic parameters in faba bean crop. Under drought stress, values of biomass and chlorophyll in the genotypes Nubaria 1, Giza 2, Giza 429, Giza 716, Nubaria 1 × Giza 716, Sakha 2 × Giza 2, Sakha 2 × Giza 716, Giza 2 × Giza 429 and Sakha 2 × Sakha 1 were higher than those of the rest of genotypes. Notably, a marked increase was recorded in the biomolecule indicators of plant oxidative stresses; proline and soluble sugars with same genotypes after water deficiency. Transcriptome analysis via real-time reverse transcriptase-polymerase chain reaction revealed that expression levels of VfDHN4,APETALA2, VfHSP18, and VfAQP2genes were altered in response to drought conditions. The results demonstrated that all genes were differentially expressed in the studied faba bean genotypes due to water shortage intensity according to cultivar's tolerance to drought. This study could be a distinctive strategy to speed up breeding programs via exploring the molecular, biochemical and physiological mechanism of drought tolerance in faba bean or other economically important crops.

DOI

10.21608/ejdr.2021.17275.1050

Keywords

Faba bean, Drought stress, proteomic, Metabolomic, genomic and transcriptomic

Authors

First Name

Mohamed

Last Name

Ewas

MiddleName

-

Affiliation

Genetics and Cytology Unit, Department of Genetic Resources, Desert Research Center, El Matareya, Cairo, Egypt

Email

mohamed82@webmail.hzau.edu.cn

City

Cairo

Orcid

-

First Name

Sayed

Last Name

Omar

MiddleName

A.

Affiliation

Plant Breeding Unit, Department of Genetic Resources, Desert Research Center, El-Matareya, Cairo, Egypt

Email

said_plantstress1959@yahoo.com

City

-

Orcid

-

First Name

Eman

Last Name

El-Sarag

MiddleName

-

Affiliation

Department of Crop Production, Faculty of Agriculture, Al-Arish University, Egypt

Email

eman_elsarag2010@live.com

City

-

Orcid

-

First Name

Mohamed

Last Name

Mubarak

MiddleName

H.

Affiliation

Department of Crop Production, Faculty of Agriculture, Al-Arish University, Egypt

Email

mobark_mohamed99@yahoo.com

City

-

Orcid

-

Volume

69

Article Issue

2

Related Issue

21036

Issue Date

2019-12-01

Receive Date

2019-09-24

Publish Date

2021-01-23

Page Start

47

Page End

67

Print ISSN

1687-8043

Online ISSN

2356-9875

Article File

EJDR_Volume 69_Issue 2_Pages 47-67.pdf

PDF . 1.7MB

Link

https://ejdr.journals.ekb.eg/article_142476.html

Detail API

https://ejdr.journals.ekb.eg/service?article_code=142476

Order

4

Type

Original Article

Type Code

117

Publication Type

Journal

Publication Title

Egyptian Journal of Desert Research

Publication Link

https://ejdr.journals.ekb.eg/

MainTitle

GENOME-WIDE CHARACTERIZATION OF DROUGHT-RESPONSIVE GENES ACCELERATES FABA BEAN BREEDING PROGRAM

Details

Type

Article

Created At

22 Jan 2023