The field work was carried out at the Experimental Farm of Desert Research Center at Siwa Oasis, Marsa Matrouh Governorate. During the two consecutive seasons of 2015/2016 and 2016/2017, the experiments were conducted to study the effect of bio-fertilizers namely Nitroben at the rate of 1.5 kg/fed, Phosphorin at the rate of 1.5 kg/fed and a mixture of them and nano-titanium dioxide (TiO₂) as foliar application at the rate of 0, 1 and 2 mg/L on growth, yield, chemical composition and pickle quality of turnip plant, purple top white glop cv. grown in sandy soil conditions. Results revealed that the highest values of growth, yield parameters and chemical contents were clear with bio-fertilizer application compared to control treatment (without application). No significant differences were found between bio-fertilizer treatments on most parameters. The highest values of growth, yield parameters and chemical contents were obtained with TiO₂application at the rate 2 mg/L, followed by rate of 1 mg/L as compared to control treatment (without application). No significant differences were found among TiO₂nanoparticle treatmentson most parameters. Regarding to the effect of the bio-fertilizers and TiO₂treatments on turnip pickle quality, it was found that, Nitroben and Phosphorin bio-fertilizers and TiO₂spray had the more acceptable sensory scores and excellent texture characteristics. Also, investment ratio had been calculated.