The study aimed to evaluate the antimicrobial and antioxidant abilities of four endophytic actinomycetes (strain Act₁, Act₂, Act₃, and Act₄) isolated from leaves of Asphodelus tenuifolius, as well as assessment of their phytochemical profile. Referring to morphological characters and 16S rDNA sequence analysis, actinomycete (strain Act2) was identified as Streptomyces fenghuangensis (GIMN4.003T) with 96% identical sequence and accession number (NR1175021). The results showed variant inhibitory activity against pathogenic bacteria and fungi. Act₂endophytic actinomycete isolate showed broad spectrum activity against all pathogenic tested organisms, recording notable MIC value 10 µg/ml against Bacillus subtilus andBacillus cereus, while At₃and At₄showed strong impact on Candida albicans. All of the ethyl acatate extracts of actinomycete isolates revealed high antioxidant activity ranging from 45-82% using DPPH radical. Phytochemical screening of the isolates indicates the presence of phenolics, flavonoids, sterols, anthraquinones and naphthaquinones.